한국초파리연구자원포탈(Korea Drosophila Resource Portal)
한국초파리연구자원포탈(Korea Drosophila Resource Portal)

Copyright © KMPC. All Rights Reserved.

08826 서울특별시 관악구 관악로 1 서울대학교 생명공학연구동(81동) 520호 국가마우스표현형분석사업단
사업자번호 119-82-11812|대표 성제경

+82-2-885-8393 animalmodel@snu.ac.kr
top

Service

Home > Service > Injection Guide

Injection Guide
Injection Guide
Service list
Services Charge Shipment Etc
P-element injection 200 100 larvae -
300 < 5 transformants -
Site-directed injection
(Phi31 landing site) 		200 100 larvae -
300 < 5 transformants -
BAC injection ≧ 400 1-2 transformants Depending on Construct Size
CRISPR injection 300 larvae 300 injections
  • · A handling fee (50,000 won) will be charged per order.
  • · If the larva survival rate after injection is significantly low, additional charge may be incurred.
  • · For CRISPR knock-in injection, please proceed after inquiry (kdrc@gist.ac.kr).
  • · DNA Requirements: Concentration (500ng/ul or more) and the amount (50ul or more)
  • · For fast processing, Qiagen-column quality DNA is required.
Available phiC31 attP-bearing stocks
- KDRC strains (mainly used)
Stock# Symbol Chr Cyto Location Genotype
26 72a 2R: 6390660 47C3 intergenic w- (I); Landing site 72a (II)
26 72a 2R: 6390660 47C3 intergenic w- (I); Landing site 72a (II)
26 72a 2R: 6390660 47C3 intergenic w- (I); Landing site 72a (II)
- BDSC strains (processing schedule extended)
Stock# Symbol Chr Cyto Genotype
9726 PBac{y[+]-attP-9A}VK00006 1 19E7 y[1] w[1118] PBac{y[+]-attP-9A}VK00006
9723 PBac{y[+]-attP-3B}VK00002 2 28E7 y[1] w[1118]; PBac{y[+]-attP-3B}VK00002
9732 PBac{y[+]-attP-9A}VK00013 3 76A2 y[1] w[1118]; PBac{y[+]-attP-9A}VK00013
9738 PBac{y[+]-attP-9A}VK00020 3 99F8 y[1] w[1118]; PBac{y[+]-attP-9A}VK00020
9750 PBac{y[+]-attP-3B}VK00033 3 65B2 y[1] w[1118]; PBac{y[+]-attP-3B}VK00033
9752 PBac{y[+]-attP-3B}VK00037 2 22A3 y[1] w[1118]; PBac{y[+]-attP-3B}VK00037
9755 PBac{y[+]-attP-3B}VK00040 3 87B10 y[1] w[1118]; PBac{y[+]-attP-3B}VK00040/TM6B, Tb[+]
25709 P{CaryP}attP40 2 25C6 y[1] v[1] P{y[+t7.7]=nos-phiC31int.NLS}X; P{y[+t7.7]=CaryP}attP40
25710 P{CaryP}attP2 3 68A4 y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31int.NLS}X; P{y[+t7.7]=CaryP}attP2
32233 P{CaryIP}su(Hw)attP8 1 8E10 y[1] w[*] P{y[+t7.7]=CaryIP}su(Hw)attP8