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K-Gut Project
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| Crop | PV | R1 | R2 | R3 | R4 | R5 | MHJ (mid-hind junction) |
Hindgut (illeum) |
Rectum |
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K-GUT Project
K-GUT project is an on-going project of KDRC, which aims to archive Drosophila gut GAL4 transgenes and their expression patterns. K-GUT project provides a database accessible to all researchers in the Drosophila community.
Principal Investigators Participating in K-GUT Project
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Greg S. B. Suh
- KAIST
- seongbaesuh@kaist.ac.kr
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Jae Young Kwon
- Sungkyunkwan University
- jykwon@skku.edu
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Won-Jae Lee
- Seoul National University
- lwj@snu.ac.kr
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Young-Joon Kim
- GIST
- kimyj@gist.ac.kr
Publication
Seung Yeon Lim, Hyejin You, Jinhyeong Lee, Jaejin Lee, Yoojin Lee, Kyung-Ah Lee, Boram Kim, Ji-Hoon Lee, JiHyeon Jeong, Sooin Jang, Byoungsoo Kim, Hyungjun Choi, Gayoung Hwang, Min Sung Choi, Sung-Eun Yoon, Jae Young Kwon, Won-Jae Lee, Young-Joon Kim & Greg S. B. Suh (2020) Identification and characterization of GAL4 drivers that mark distinct cell types and regions in the Drosophila adult gut, Journal of Neurogenetics, DOI: 10.1080/01677063.2020.1853722
For reprint requests, kdrc@gist.ac.kr
Imaging Conditions
| K-GUT ID | GS | JK | WL | LS |
|---|---|---|---|---|
| Microscopy | Zeiss LSM 780 | Zeiss LSM 700 | Zeiss LSM 780 | Zeiss Lightsheet Z.1 |
| Lens | 20X (Plan-Apochromat 20x/0.8 M27) |
20X | 20X (Plan Apochromat 20x/0.8) |
10X (W Plan-Apochromat 10x/0.5) |
| Antibodies | GFP (Invitrogen, A11122) | GFP (Invitrogen, A11122) | GFP (Invitrogen, A11122) | GFP (Invitrogen, A6455) |
| Prospero (DSHB, MR1A) | Prospero (DSHB, MR1A) | Prospero (DSHB, MR1A) | ||
| AlexaFluor-488 (Invitrogen, A21206) |
AlexaFluor-488 (Invitrogen, A11034) |
AlexaFluor-488 (Invitrogen, A11008) |
AlexaFluor-488 (Invitrogen, A32723) |
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| AlexaFluor-594 (Invitrogen, A21203) |
AlexaFluor-568 (Invitrogen, A11031) |
AlexaFluor-568 (Invitrogen, A11004) |
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| DAPI (Sigma, D9542) | DAPI (Unkown) | DAPI (Sigma, D9542) | Hoechst (Invitrogen, H3570) | |
| Palloidin | AlexaFluor-555 Phalloidin (Invitrogen, A34055) |
- · KGP 1.1.3 - Released April 12, 2023 - Add 19 Gal4 and Split-Gal4 Data and 45 Images
- · KGP 1.1.2 - Released May 18, 2022 - Add 360 Gal4 Data and 1,121 Images
- · KGP 1.1.1 - Released May 27, 2021 - Add 105 Split-Gal4 Data and Images
- · KGP 1.1.0 - Released December 23, 2020 - Add 199 Light-sheet images
- · KGP 1.0.0 - Released October 28, 2020
Total Images: 0
Dr. Sung-Eun Yoon (KDRC, kdrc@gist.ac.kr)
